Integrated approach on azo dyes degradation using laccase enzyme and Cul nanoparticle

Abstract Azo dyes released by the textile industries cause severe damage to environment and living organisms. The degradation of azo is widely studied using enzymatic methods. Laccase a copper-containing enzyme that degrades into less toxic compounds. In this work, crude laccase produced alkaliphile Pseudomonas mendocina in mixed dye showed 0.386 U/mL activity at pH 8.5. A combination green synthesized nanoparticles was used dye. 58.4% 72 h, while photocatalytic 15.9%. copper iodide resulted 15.8% degradation. However, it noticed combined method involving both CuI gave 62.3% 60 min. Interaction with silico analysis predicted binding energy reactive red (−7.19 kcal/mol), brown (−8.57 black (−9.17 kcal/mol) respectively.